The 2000 AF2 season was the very first season of the AF2. The league champions were the Quad City Steamwheelers, who capped off a perfect season with a victory over the Tennessee Valley Vipers in ArenaCup I. This is the only AF2 season lacking division alignment.
| Team | Overall | Conference | ||||
|---|---|---|---|---|---|---|
| Wins | Losses | Percentage | Wins | Losses | Percentage | |
| American Conference | ||||||
| Augusta Stallions | 13 | 3 | 0.812 | 10 | 2 | 0.833 |
| Norfolk Nighthawks | 10 | 6 | 0.625 | 9 | 4 | 0.600 |
| Jacksonville Tomcats | 9 | 7 | 0.562 | 5 | 4 | 0.556 |
| Carolina Rhinos | 9 | 7 | 0.562 | 5 | 4 | 0.556 |
| Richmond Speed | 7 | 9 | 0.437 | 4 | 3 | 0.571 |
| Charleston Swamp Foxes | 4 | 12 | 0.333 | 3 | 7 | 0.300 |
| Greensboro Prowlers | 3 | 13 | 0.187 | 0 | 8 | 0.167 |
| Roanoke Steam | 3 | 13 | 0.187 | 0 | 8 | 0.167 |
| National Conference | ||||||
| Quad City Steamwheelers | 16 | 0 | 1.000 | 11 | 0 | 1.000 |
| Tennessee Valley Vipers | 10 | 6 | 0.625 | 6 | 3 | 0.667 |
| Tulsa Talons | 9 | 7 | 0.562 | 5 | 7 | 0.417 |
| Pensacola Barracudas | 8 | 8 | 0.500 | 6 | 3 | 0.667 |
| Birmingham Steeldogs | 7 | 9 | 0.437 | 3 | 7 | 0.300 |
| Arkansas Twisters | 7 | 9 | 0.437 | 2 | 7 | 0.222 |
| Tallahassee Thunder | 5 | 11 | 0.312 | 1 | 7 | 0.125 |
| Round 1 | Semifinals | ArenaCup I | |||||||||||
| 8 | Pensacola | 19 | |||||||||||
| 1 | Quad City | 55 | |||||||||||
| 4 | Norfolk | 27 | |||||||||||
| 1 | Quad City | 75 | |||||||||||
| 5 | Jacksonville | 28 | |||||||||||
| 4 | Norfolk | 41 | |||||||||||
| 3 | Tennessee Valley | 59 | |||||||||||
| 1 | Quad City | 68 | |||||||||||
| 7 | Carolina | 64 | |||||||||||
| 2 | Augusta | 76 | |||||||||||
| 3 | Tennessee Valley | 47 | |||||||||||
| 2 | Augusta | 40 | |||||||||||
| 6 | Tulsa | 28 | |||||||||||
| 3 | Tennessee Valley | 49 | |||||||||||
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